منابع مشابه
Serial Femtosecond Crystallography
X-ray free-electron lasers produce brief flashes of X-rays that are of about a billion times higher peak brightness than achievable from storage ring sources. Such a tremendous jump in X-ray source capabilities, that came in 2009 when the Linac Coherent Light Source begun operations, was unprecedented in the history of X-ray science. Protein structure determination through the method of macromo...
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Serial crystallography should be possible with a much wider range of radiation sources as Ayyer et al. [IUCrJ (2015), 2, 29-34] show that crystallographic intensities can be recovered from randomly oriented frames which are too sparse for indexing.
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A brief history is given of how X-ray diffraction data from crystals have been recorded. Today there are new possibilities, spawned by the availability of free electron lasers that produce powerful femtosecond long X-ray pulses.
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A synopsis of and prospects for de novo phasing using diffraction data collected at X-ray free-electron lasers are given.
متن کاملBreaking the indexing ambiguity in serial crystallography.
In serial crystallography, a very incomplete partial data set is obtained from each diffraction experiment (a `snapshot'). In some space groups, an indexing ambiguity exists which requires that the indexing mode of each snapshot needs to be established with respect to a reference data set. In the absence of such re-indexing information, crystallographers have thus far resorted to a straight mer...
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ژورنال
عنوان ژورنال: Crystals
سال: 2020
ISSN: 2073-4352
DOI: 10.3390/cryst10100854